Why do you NOT add the indole until after incubation for the SIM medium

For Ex. 8-5 (Blood Typing):

1. Do you know your blood type? If so, what is it (optional disclosure)?

2. What are two blood borne diseases that someone in lab might have that make testing student blood an inappropriate activity?

3. What is an antigen? What is an antibody?

4. What is hemeagglutination?

Exercise 8-5

1. What antigens are present in a person with typeAB blood? What antibodies are possibly present?

2. Is it possible for a person with type A blood and a person with type B blood to have a child with type O blood? Explain your answer.

3. What blood type is commonly referred to a being a “universal donor”? Can a person with this blood type actually safely donate blood to anyone (ignore disease factors and answer based on blood typing factors)?

4. Why do we discuss ABO blood type and Rh blood type as separate tests?

5. Based on the information in Table 8-3 in you lab manual (pg. 531), what percentage of Whites have at least one A allele? What percentage of Hispanics have at least one B allele?

6. What factors might cause the class data to be different from the US averages for blood type distribution?

For Ex. 5-18 (SIM medium):

For Ex. 5-21 (Blood Agar):

1. Why do you NOT add the indole until after incubation for the SIM medium?

2. What molecule is broken down by the tryptophanase enzyme? Which kind of molecule is it?

3. Which type of hemolysis results in complete destruction of the red blood cell? How do we know that they have been destroyed?

4. Why do you streak the surface and stab the blood agar?