What physical characteristic of the lipids makes mixing them into the agar difficult

Chapter 24: Microbial Diseases of the Urinary and Reproductive Systems
For each of the diseases identified in this chapter:
· identify the scientific name of the causative pathogenic organism
· identify the type of organism (bacterium, fungus, virus, protozoan, helminth, prion, etc.)
· identify the key signs & symptoms of the disease (especially those that differentiate it from other similar diseases)
· describe differentiating features of the disease: pathogenesis of the disease including any common vectors, any common means of contraction, any individuals that are more likely to contract the disease, etc.
Bacterial Diseases of the Urinary System
2. leptospirosis
Nonveneral Diseases of the Reproductive System
6. vaginal candidiasis
Sexually Transmitted Infections (STIs) and Diseases (STDs)
Bacterial STDs
8. syphilis
9. chlamydial infections
Viral STDs
11. genital herpes
12. genital warts
Protozoan STDs
13. trichomoniasis


LAB Book Used: Leboffe and Pierce (2016). Brief microbiology laboratory theory and application (3rd ed.) Englewood, CO: Morton Publishing. ISBN: 9781617314773

Exercise 5-12

1. What is tributyrin?

2. What physical characteristic of the lipids makes mixing them into the agar difficult?

3. These plates are supposed to be observed within 3 days of preparation. What characteristic of the plates causes this requirement?

4. If glucose were to be accidentally added to a starch plate, extended incubation might reveal starch usage. Would that be true of the lipid hydrolysis test?

5. Sue deMonas observed a negative result on her plates on Tuesday, the day after she prepared the plates and inoculated them. The next Tuesday she went to take a picture of the result to show her mom and the entire plate was clear. What conclusion should Sue make about her observations?

6. Does growth on this agar mean that the organism has used the lipids? Explain.

7. What possible ways can the cell use to recover the NAD+ used in the lipid hydrolysis pathways?

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