What enzyme is used to break down the starch in the starch agar

Exercise 5-7
1. Citrate can be transported into the cell and used for energy metabolism. What enzyme is required for citrate transport? Why do cell that do not have this enzyme fail to grow on the medium?
2. What is the approximate pH of uninoculated citrate agar? Why does the loss of citrate affect the pH of the medium? What metabolic byproducts are made that also affect the pH of the medium?
3. Does the presence of ability to do the Krebs cycle in a cell indicate whether the cell will be able to use the citrate agar? Explain.
Exercise 5-10
4. Why is it better to let the starch plates incubate longer than the prescribed time and not less than the prescribed time? Explain in terms of enzymes and substrate (what happens in the plate during incubation). Explain how this relates to the addition of iodine only after incubation in the starch plate.
5. What enzyme is used to break down the starch in the starch agar? Is this an exoenzyme or an endoenzyme? Explain your thinking.
6. Why is glucose never an ingredient of starch agar?

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