What are the two types of agranulocytes

Exercise 5-8

1. The color of the medium can change over time – from the original purple to a yellow color and back to purple. What processes cause each color change to occur?

2. What are the two products of lysine decarboxylase? Arginine and ornithine decarboxylase tests work in the same manner as the lysine decarboxylase test. How can tests for different enzymes work in the same way?

3. Why does this test require a week of incubation? What might happen if you observed the results after only 24-48 hours?

Exercise 5-13

1. Milk contains lots of lactose? Why would an organism want to break down casease in milk?

2. Would you think most milk-spoilage organisms make casease? Explain. Would this necessarily be true of pathogens found in milk?

3. What are two ways in which casease is similar to amylase? How is it different?

For Ex. 5-14 (Gelatin Hydrolysis):

For Ex. 6-4 (Differential Blood Cell Count):

1. What type of macromolecule is broken down by gelatinase?

2. Based on the structure of gelatin, where does the gelatinase enzyme do its work? What kind of enzyme is this?

3. What is the meaning of “leukocyte?” What is an erythrocyte? Which is more prevalent in normal human blood?

4. What is a “landmark” cell and why should you choose one when beginning your count?

Exercise 5-14

1. Why must the gelatin tubes be cold before you can properly observe the tubes to determine the result?

2. What benefit would we have obtained by using a control in this experiment? Assume you have been doing this test for years and know exactly what the positive and negative look like, so that is not why you did the control.

Exercise 6-5

1. What are the three types of granulocytes? What is a distinguishing feature of each type (what lets you identify the cell as that type of granulocyte?)?

2. What are the two types of agranulocytes? How are these cells different from granulocytes? What is a distinguishing feature of each agranulocyte type (what lets you identify the cell as that type of agranulocyte?)?

3. Did your percentages of each type of WBC agree with the expected percentages? If not, what may have led to the difference (assuming the sample is from a healthy person)? If they did agree, can you make any conclusions?

4. Why is differential blood count done in ‘real life?’ What disease conditions might be detected?