How would you know this based on your DNA fingerprint results

 You have been given several bacterial strain from various countries around the world. It is thought that all the strains cause the same gastrointestinal illness and are genetically identical. After carrying out a DNA fingerprint analysis of the strains, you find that four different strain types are present. How would you know this based on your DNA fingerprint results? Explain. Explain how you could use DNA-DNA hybridization and multilocus sequence typing to determine if these four strains would belong to the same species. Would you also be able to use a G-C ratio to determine if they were the same species? Why or why not? Explain.
6. In order for the Gram stain to work properly the ethanol in the decolorizer must be at a concentration greater than 60%. Imagine that you are working in the microbiology laboratory. You are instructed to make more Gram stain reagents. You mistakenly prepare a 50% ethanol solution. How will this affect the results of your Gram stains? Explain. You realize your mistake and prepare the decolorizer using the proper concentration of ethanol. The post-doc in the lab tells you about the Mycoplasma bacteria. These are bacteria that are taxonomically related to the Gram positive bacteria but do not have a cell wall. You are curious and decide to conduct a Gram stain on the Mycoplasma that you have in the lab. What result will you observe? Explain why.

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