How would a strain of Escherichia coli look under a microscope if it had a mutation that inactivated the protein MreB compared to a strain that had a functional MreB

15. How would a strain of Escherichia coli look under a microscope if it had a mutation that inactivated the protein MreB compared to a strain that had a functional MreB? Why does this happen? Would this effect cell division in the mutant strain? Explain. Which Fts protein has a similar cell division roll to MreB? Explain. Hypothesize the problems a mutant bacterium would have if they were unable to produce functional Min proteins. Would this mutant be easy or difficult to isolate and grow? Explain.
16. Some Gram negative bacterial cells are considered non-autolytic, which means they will not lyse or break down immediately after cell death. Diagram and label a growth curve comparing the log of the viable cells over time and a growth curve of the optical density of the sample over time. Explain the similarities and differences your would see between the two curves.
17. Listeria monocytogenes infections are often transmitted through contaminated food. A person who has contracted listeriosis usually has a high fever, muscle aches, and gastrointestinal problems such as diarrhea. In severe cases meningitis can develop and the disease can be deadly. Refrigeration of food contaminated with this microbe does not prevent the transmission of the disease as the microbe can grow in refrigerator temperatures. How would you categorize Listeria based on its ability to grow in the refrigerator? Explain. Which temperature category would most pathogens fall into? Explain. Which temperature category or categories would you not expect to find any pathogen? Explain.

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