Differentiatethemicroscopicmorphologyofstaphylococciandstreptococciasseenby Gramstain
EXERCISE3-TheEnterobacteriaceae(EntericBacilli)
1. Whatdoestheterm IMViCmean?
2. Whyis theIMViCusefulinidentifyingEnterobacteriaceae?Arefurtherbiochemicaltests necessaryforcompleteidentification?
3. WhatdiagnostictestdifferentiatesProteusandProvidenciaspeciesfrom other
Enterobacteriaceae?
4. HowisE.colidistinguishedfrom P. vulgarisonMacConkeyagar?OnaTSIslant?
5. Whyisitimportanttodifferentiateglucosenonfermentersfrom Enterobacteriaceae?
EXERCISE4–Streptococci,Pneumococci,and Enterococci
1. Differentiatethemicroscopicmorphologyofstreptococciandpneumococciasseenby
Gramstain.
2. Whattypeof hemolysisis producedbyS. pneumoniae?
3. HowisS.pneumoniaedistinguishedfrom otherstreptococciwiththesamehemolytic properties?
4. Describethehemolysisproducedbyalpha-hemolytic,beta-hemolytic,andnonhemolytic streptococci.
5. Whattypeofhemolysisis displayedbythegroupablestreptococcithataremostpathogenic forhumanbeings?
EXERCISE5-UrineCultureTechniques
1. Whatis bacteriuria?Whenis itsignificant?
2. Howdomicroorganismsentertheurinarytract?
3. Whyisasepticurinecollectionimportantwhenculturesareordered?
4. Listfivebacteriathatcancauseurinarytractinfection.
5. Ifyoucounted20 coloniesfrom a0.01-mlinoculumofa1:10dilutionofurine,howmany organismspermilliliterof specimenwouldyoureport?Isthisnumbersignificant?
Week10ReviewSheet II
EXERCISE1-SerodiagnosisofInfectiousDisease
1. Defineserumtiter.
2. Whatareacuteandconvalescentsera?Whymustbothbetestedtomakeaserological diagnosisof infectiousdisease?
3. Whatis thedifferencebetweenanagglutinationtestanda precipitationtest?
4. Ina pairedserumsample,whattestresultsindicaterecentinfection?
5. Whatis ahumoralantibody?
EXERCISE2-CulturingMicroorganismsfromtheEnvironment
1. Whydomicrobiologistswearlaboratorycoats?Didyouconfirmthatthisisnecessary?
2. Whyisitnecessarytowearclean,protectiveclothingwhencaringforapatient?
3. Whyshouldhairbekeptcleanandoutofthewaywhencaringforpatients?
4. Howcanthenumberofmicroorganismsintheenvironmentbecontrolled?
5. Whenandwhyis handwashingimportantinpatientcare?
6. Howcanthosewhocareforpatientsavoidspreadingmicroorganismsamongthem?
EXERCISE3-Staphylococci
1. Differentiatethemicroscopicmorphologyofstaphylococciandstreptococciasseenby
Gramstain.
2. Whatarethetwotypesofstaphylococcalcoagulase?
3. Whatis proteinA?Describeonemethodofdetectingit.
4. Whatpropertiesof S.aureusdistinguishitfrom S.epidermidisandS.saprophyticus?
5. Whyarestaphylococcalinfectionsfrequentamonghospitalpatients?
__________________________________________________________________________
Must have APA citation and include questions and answers.
EXERCISE3-TheEnterobacteriaceae(EntericBacilli)
1. Whatdoestheterm IMViCmean?
2. Whyis theIMViCusefulinidentifyingEnterobacteriaceae?Arefurtherbiochemicaltests necessaryforcompleteidentification?
3. WhatdiagnostictestdifferentiatesProteusandProvidenciaspeciesfrom other
Enterobacteriaceae?
4. HowisE.colidistinguishedfrom P. vulgarisonMacConkeyagar?OnaTSIslant?
5. Whyisitimportanttodifferentiateglucosenonfermentersfrom Enterobacteriaceae?
EXERCISE4–Streptococci,Pneumococci,and Enterococci
1. Differentiatethemicroscopicmorphologyofstreptococciandpneumococciasseenby
Gramstain.
2. Whattypeof hemolysisis producedbyS. pneumoniae?
3. HowisS.pneumoniaedistinguishedfrom otherstreptococciwiththesamehemolytic properties?
4. Describethehemolysisproducedbyalpha-hemolytic,beta-hemolytic,andnonhemolytic streptococci.
5. Whattypeofhemolysisis displayedbythegroupablestreptococcithataremostpathogenic forhumanbeings?
EXERCISE5-UrineCultureTechniques
1. Whatis bacteriuria?Whenis itsignificant?
2. Howdomicroorganismsentertheurinarytract?
3. Whyisasepticurinecollectionimportantwhenculturesareordered?
4. Listfivebacteriathatcancauseurinarytractinfection.
5. Ifyoucounted20 coloniesfrom a0.01-mlinoculumofa1:10dilutionofurine,howmany organismspermilliliterof specimenwouldyoureport?Isthisnumbersignificant?
Week10ReviewSheet II
EXERCISE1-SerodiagnosisofInfectiousDisease
1. Defineserumtiter.
2. Whatareacuteandconvalescentsera?Whymustbothbetestedtomakeaserological diagnosisof infectiousdisease?
3. Whatis thedifferencebetweenanagglutinationtestanda precipitationtest?
4. Ina pairedserumsample,whattestresultsindicaterecentinfection?
5. Whatis ahumoralantibody?
EXERCISE2-CulturingMicroorganismsfromtheEnvironment
1. Whydomicrobiologistswearlaboratorycoats?Didyouconfirmthatthisisnecessary?
2. Whyisitnecessarytowearclean,protectiveclothingwhencaringforapatient?
3. Whyshouldhairbekeptcleanandoutofthewaywhencaringforpatients?
4. Howcanthenumberofmicroorganismsintheenvironmentbecontrolled?
5. Whenandwhyis handwashingimportantinpatientcare?
6. Howcanthosewhocareforpatientsavoidspreadingmicroorganismsamongthem?
EXERCISE3-Staphylococci
1. Differentiatethemicroscopicmorphologyofstaphylococciandstreptococciasseenby
Gramstain.
2. Whatarethetwotypesofstaphylococcalcoagulase?
3. Whatis proteinA?Describeonemethodofdetectingit.
4. Whatpropertiesof S.aureusdistinguishitfrom S.epidermidisandS.saprophyticus?
5. Whyarestaphylococcalinfectionsfrequentamonghospitalpatients?
__________________________________________________________________________
Must have APA citation and include questions and answers.
