Differentiatethemicroscopicmorphologyofstaphylococciandstreptococciasseenby Gramstain

EXERCISE3-TheEnterobacteriaceae(EntericBacilli)
 
 
1.   Whatdoestheterm IMViCmean?
 
2.   Whyis theIMViCusefulinidentifyingEnterobacteriaceae?Arefurtherbiochemicaltests necessaryforcompleteidentification?
 
3.   WhatdiagnostictestdifferentiatesProteusandProvidenciaspeciesfrom other
Enterobacteriaceae?
 
4.   HowisE.colidistinguishedfrom P. vulgarisonMacConkeyagar?OnaTSIslant?
 
5.   Whyisitimportanttodifferentiateglucosenonfermentersfrom Enterobacteriaceae?
 
EXERCISE4–Streptococci,Pneumococci,and Enterococci
 
1.   Differentiatethemicroscopicmorphologyofstreptococciandpneumococciasseenby
 
Gramstain.
 
2.   Whattypeof hemolysisis producedbyS. pneumoniae?
 
3.   HowisS.pneumoniaedistinguishedfrom otherstreptococciwiththesamehemolytic properties?
 
4.   Describethehemolysisproducedbyalpha-hemolytic,beta-hemolytic,andnonhemolytic streptococci.
 
5.   Whattypeofhemolysisis displayedbythegroupablestreptococcithataremostpathogenic forhumanbeings?
 
EXERCISE5-UrineCultureTechniques
 
1.   Whatis bacteriuria?Whenis itsignificant?
 
2.   Howdomicroorganismsentertheurinarytract?
 
3.   Whyisasepticurinecollectionimportantwhenculturesareordered?
 
4.   Listfivebacteriathatcancauseurinarytractinfection.
 
5.   Ifyoucounted20 coloniesfrom a0.01-mlinoculumofa1:10dilutionofurine,howmany organismspermilliliterof specimenwouldyoureport?Isthisnumbersignificant?
 

 

 
Week10ReviewSheet II
 
EXERCISE1-SerodiagnosisofInfectiousDisease
 
1.   Defineserumtiter.
 
2.   Whatareacuteandconvalescentsera?Whymustbothbetestedtomakeaserological diagnosisof infectiousdisease?
 
3.   Whatis thedifferencebetweenanagglutinationtestanda precipitationtest?
 
4.   Ina pairedserumsample,whattestresultsindicaterecentinfection?
 
5.   Whatis ahumoralantibody?
 
EXERCISE2-CulturingMicroorganismsfromtheEnvironment
 
1.   Whydomicrobiologistswearlaboratorycoats?Didyouconfirmthatthisisnecessary?
 
2.   Whyisitnecessarytowearclean,protectiveclothingwhencaringforapatient?
 
3.   Whyshouldhairbekeptcleanandoutofthewaywhencaringforpatients?
 
4.   Howcanthenumberofmicroorganismsintheenvironmentbecontrolled?
 
5.   Whenandwhyis handwashingimportantinpatientcare?
 
6.   Howcanthosewhocareforpatientsavoidspreadingmicroorganismsamongthem?
 
EXERCISE3-Staphylococci
 
1.   Differentiatethemicroscopicmorphologyofstaphylococciandstreptococciasseenby
Gramstain.
 
2.   Whatarethetwotypesofstaphylococcalcoagulase?
 
3.   Whatis proteinA?Describeonemethodofdetectingit.
 
4.   Whatpropertiesof S.aureusdistinguishitfrom S.epidermidisandS.saprophyticus?
 
5.   Whyarestaphylococcalinfectionsfrequentamonghospitalpatients?
__________________________________________________________________________
 
Must have APA citation and include questions and answers.
EXERCISE3-TheEnterobacteriaceae(EntericBacilli)
 
 
1.   Whatdoestheterm IMViCmean?
 
2.   Whyis theIMViCusefulinidentifyingEnterobacteriaceae?Arefurtherbiochemicaltests necessaryforcompleteidentification?
 
3.   WhatdiagnostictestdifferentiatesProteusandProvidenciaspeciesfrom other
Enterobacteriaceae?
 
4.   HowisE.colidistinguishedfrom P. vulgarisonMacConkeyagar?OnaTSIslant?
 
5.   Whyisitimportanttodifferentiateglucosenonfermentersfrom Enterobacteriaceae?
 
EXERCISE4–Streptococci,Pneumococci,and Enterococci
 
1.   Differentiatethemicroscopicmorphologyofstreptococciandpneumococciasseenby
 
Gramstain.
 
2.   Whattypeof hemolysisis producedbyS. pneumoniae?
 
3.   HowisS.pneumoniaedistinguishedfrom otherstreptococciwiththesamehemolytic properties?
 
4.   Describethehemolysisproducedbyalpha-hemolytic,beta-hemolytic,andnonhemolytic streptococci.
 
5.   Whattypeofhemolysisis displayedbythegroupablestreptococcithataremostpathogenic forhumanbeings?
 
EXERCISE5-UrineCultureTechniques
 
1.   Whatis bacteriuria?Whenis itsignificant?
 
2.   Howdomicroorganismsentertheurinarytract?
 
3.   Whyisasepticurinecollectionimportantwhenculturesareordered?
 
4.   Listfivebacteriathatcancauseurinarytractinfection.
 
5.   Ifyoucounted20 coloniesfrom a0.01-mlinoculumofa1:10dilutionofurine,howmany organismspermilliliterof specimenwouldyoureport?Isthisnumbersignificant?
 

 

 
Week10ReviewSheet II
 
EXERCISE1-SerodiagnosisofInfectiousDisease
 
1.   Defineserumtiter.
 
2.   Whatareacuteandconvalescentsera?Whymustbothbetestedtomakeaserological diagnosisof infectiousdisease?
 
3.   Whatis thedifferencebetweenanagglutinationtestanda precipitationtest?
 
4.   Ina pairedserumsample,whattestresultsindicaterecentinfection?
 
5.   Whatis ahumoralantibody?
 
EXERCISE2-CulturingMicroorganismsfromtheEnvironment
 
1.   Whydomicrobiologistswearlaboratorycoats?Didyouconfirmthatthisisnecessary?
 
2.   Whyisitnecessarytowearclean,protectiveclothingwhencaringforapatient?
 
3.   Whyshouldhairbekeptcleanandoutofthewaywhencaringforpatients?
 
4.   Howcanthenumberofmicroorganismsintheenvironmentbecontrolled?
 
5.   Whenandwhyis handwashingimportantinpatientcare?
 
6.   Howcanthosewhocareforpatientsavoidspreadingmicroorganismsamongthem?
 
EXERCISE3-Staphylococci
 
1.   Differentiatethemicroscopicmorphologyofstaphylococciandstreptococciasseenby
Gramstain.
 
2.   Whatarethetwotypesofstaphylococcalcoagulase?
 
3.   Whatis proteinA?Describeonemethodofdetectingit.
 
4.   Whatpropertiesof S.aureusdistinguishitfrom S.epidermidisandS.saprophyticus?
 
5.   Whyarestaphylococcalinfectionsfrequentamonghospitalpatients?
__________________________________________________________________________
 
Must have APA citation and include questions and answers.

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