Bauman, R.W. (2015). Microbiology with diseases by body system (4th ed.) San Francisco: Pearson Benjamin Cummings. ISBN: 9780321918383
Chapter 17: Immunization and Immune Testing
1. Describe the difference between active vs passive immunity. Describe the difference between acquired and natural immunity. Be able to combine these four concepts correctly and provide examples of each.
2. Describe Edward Jenner’s 1796 experiment and its key finding. Also, describe where scientists got the term vaccination. Lastly, define vaccination, variolation, vaccine and immunization.
3. Identify the pros and cons and the difference between the following types of vaccines: attenuated vaccines, inactivated vaccines, toxoid vaccines, combination vaccines, and vaccines using recombinant gene technology.
4. Describe herd immunity and its purpose. Explain what is used in the U.S. to enable herd immunity.
5. Describe the three major safety concerns or problems with vaccines. Also, explain whether current research supports the claims that vaccines can trigger autism, diabetes and/or asthma.
6. Describe passive immunotherapy. Describe how antibodies are acquired for passive immunotherapy. Describe the three major limitations of passive immunotherapy.
7. Describe and identify the purpose of hybridomas and monoclonal antibodies.
Serological Tests That Use Antigens and Corresponding Antibodies
8. Describe serology and the purpose of serological tests.
10. Describe immune complexes and the basic process of precipitation and immunodiffusion tests. Also, explain the purpose of using these tests (i.e., what can they tell you?).
12. Describe and identify the purpose of agglutination tests. Also, define the following: agglutination, hemagglutination, titration and titer.
13. Describe and identify the purpose of the two types of neutralization tests.
14. Describe and identify the purpose of labeled antibody tests. Also, describe the difference between the four types of antibody tests. Lastly, be sure to identify the purpose of a Western blot test and briefly describe how it is performed.
15. Describe immunofiltration assays and immunochromatographic assays. Also, identify how they are generally different from the other tests described in the chapter.